A rapid procedure to detect the autolysin phenotype inStreptococcus pneumoniae
نویسندگان
چکیده
منابع مشابه
Cloning of Minor Autolysin of Streptococcus Pneumoniae
Abstract Background and Objective: Increased antibiotic resistant strains and inadequacy of current vaccines against pneumococcal infections necessitate the study of novel protein antigens. It seems that minor autolysin of Streptococcus pneumoniae may have antigenicity. Thus, we aimed at cloning its gene for the first time. Material and Methods: After DNA extraction of Streptococcus pneumoniae ...
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A Streptococcus (Diplococcus) pneumoniae autolysin, partially purified from cellular autolysates, was optimally active at pH 7.0 and was stimulated by monovalent cations. Addition of autolysin to walls resulted in the appearance of only N-terminal l-alanine, whereas no glycosidase activity was observed. Walls which had been solubilized by autolysin were separated by gel filtration into a low-mo...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1985
ISSN: 0378-1097,1574-6968
DOI: 10.1111/j.1574-6968.1985.tb00839.x